|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||Assay Dependent|
|Western Blot (WB)||See 2 publications below|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Rat Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from an internal region of the human Claudin-18 protein, which differs from mouse by one conservative amino acid replacement.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
Claudin-18 is a tight junction protein expressed as lung-and stomach-specific isoforms which are generated by alternative splicing of the claudin-18 gene. The lung-specific form is a downstream target gene regulated by the T/EBP/NKX2.1 transcription factor. A splice variant lacking the C-terminal cytoplasmic domain also exists in mouse, but has not been confirmed in human. Human claudin-18 demonstrates 88% amino acid sequence identity to the mouse protein. Immunohistochemical studies have demonstrated complete membrane localization of claudin-18 in lung and stomach epithelial cells, while electron microscopy has shown that it is concentrated in the cell-cell borders of these cells. These features suggest a potentially important role for claudin-18 in the structure and function of tight junctions in the lung and stomach. Claudin-18 expression has also been reported in the inner ear. Because of its highly restricted expression pattern in normal tissues and frequent activation in numerous human cancers, claudin-18 may represent an important target for antibody therapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Claudin-18; CLDN18; pulmonary associated protein J; SFTA5; SFTPJ; surfactant; surfactant associated 5; surfactant associated protein J; surfactant, pulmonary associated protein J
Gene Aliases: CLDN18; SFTA5; SFTPJ; UNQ778/PRO1572
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