|ELISA (ELISA)||Assay dependent.|
|Immunohistochemistry (Frozen) (IHC (F))||1:20|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Dog|
|Host / Isotype||Mouse / IgM|
|Immunogen||Collagen type IV.|
|Storage conditions||4° C|
This antibody does not cross-react with Collagens I, II, III, or V.
For immunohistochemistry on frozen sections, fixation with acetone for 5 minutes at -20°C is recommended. Immunohistochemistry on paraffin sections has worked, but is less effective than staining on frozen tissues.
Reconstitute with 1 ml of distilled water. Store reconstituted product at 4°C.
Collagen IV is a major constituent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio. It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: arresten; COL4A1 NC1 domain; Collagen alpha-1(IV) chain; collagen IV, alpha-1 polypeptide; collagen of basement membrane, alpha-1 chain; collagen, type IV, alpha 1
Gene Aliases: BSVD; COL4A1; RATOR
UniProt ID: (Human) P02462
Entrez Gene ID: (Human) 1282
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