|Functional Assay (FN)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:50|
|Western Blot (WB)||1:50|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Neo-epitope on C5a.|
|Storage buffer||PBS with 0.1% BSA|
|Storage conditions||4° C|
MA1-40161 detects C5a/C5a des-Arg from human samples.
MA1-40161 has been successfully used in Western blot, ELISA, immunohistochemistry (frozen), and functional assay applications. By Western blot, the antibody detects a band size of 14 kDa.
The MA1-40161 immunogen is the Neo-epitope on C5a.
The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to a susceptibility to liver fibrosis and to rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: anaphylatoxin C5a analog; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4; C5a anaphylatoxin; Complement C5; CPAMD4; FLJ17816; FLJ17822; MGC142298; prepro-C5
Gene Aliases: C5; C5a; C5b; C5D; CPAMD4; ECLZB
UniProt ID: (Human) P01031
Entrez Gene ID: (Human) 727
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