|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||1-2 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||2 µg/ml|
|Western Blot (WB)||See 20 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 7 publications below|
|Immunocytochemistry (ICC)||See 9 publications below|
|Immunohistochemistry (IHC)||See 9 publications below|
|Miscellaneous PubMed (MISC)||See 6 publications below|
|Immunofluorescence (IF)||See 6 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Immunohistochemistry - Free Floating (IHC (Free))||See 1 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 3 publications below|
|Tested Species reactivity||Mouse, Rat|
|Published species reactivity||Rat , Human , Mouse , Chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to a portion of the cytoplasmic loop of rat connexin 26.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
71-0500 was used in the immunofluorescence and western blot analysis to successfully detect Connexin26 in mouse liver sections and homogenate, respectively.
Gap junctions are conduits that allow the direct cell-to-cell passage of small cytoplasmic molecules, including ions, metabolic intermediates, and second messengers, and thereby mediate intercellular metabolic and electrical communication. Gap junction channels consist of connexin protein subunits, which are encoded by a multigene family. GJBs (gap-junction proteins or connexins) play crucial functional roles associated with these channels. Defects in GJB3 have been linked to erythrokeratodermia variabilis (EKV) is an autosomal dominant genodermatosis characterized by transient figurate red patches or hyperkeratosis. Mutations in GJB2 have also been associated with genetically derived hearing impairments, including autosomal recessive nonsyndromic deafness.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 26kD (connexin 26); 26kDa (connexin 26); beta 2; Connexin 26; Connexin-26; CX26; DFNA3; DFNB1; Gap junction beta-2 protein; gap junction channel protein connexin 26; gap junction membrane channel protein beta 2; gap junction protein; gap junction protein beta 2; GJB2; HID; KID; NSRD1; PPK
Gene Aliases: AI325222; Cnx26; Cx26; CXN-26; Gjb-2; Gjb2
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