|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Peptide corresponding to amino acid residues from the C-terminal region of mouse connexin 45 protein (accession # P28229, NP_032148.1), which is 100% homologous with rat and 93% homologous with human, rhesus monkey, swine and canine.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
41-5800 was used in the western blot analysis to successfully detect Connexin 45 in mouse atrium homogenate.
Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell closely packed transmembrane channels. Proteins, called connexins, purified from fractions of enriched gap junctions from different tissues differ. Connexins are designated by their molecular mass. Another system of nomenclature divides gap junction proteins into 2 categories, alpha and beta, according to sequence similarities at the nucleotide and amino acid levels. For example, CX43 is designated alpha-1 gap junction protein, whereas CX32 and CX26 are called beta-1 and beta-2 gap junction proteins, respectively. This nomenclature emphasizes that CX32 and CX26 are more homologous to each other than either of them is to CX43. Connexins have four transmembrane, three intracellular, and two extracellular regions. Different tissues express different connexins, though tissue specificities overlap, and a given tissue or cell can express several different connexins. Developmental regulation of at least some of the connexin genes has been found. Embryo implantation is regulated in part by temporally changing patterns of expression of connexins in the embryo and the maternal decidua.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Connexin 45; Connexin-45; CTC-296K1.4; Cx45; Gap junction alpha-7 protein; Gap junction gamma-1 protein; gap junction membrane channel protein alpha 7; gap junction protein, gamma 1, 45kDa
Gene Aliases: C130009G16Rik; Cnx45; CX45; Cxn-45; Gja-7; GJA7; GJC1
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