|Western Blot (WB)||1:10,000|
|Tested Species reactivity||Human, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Full length recombinant Coronin 1a expressed in and purified from E. coli.|
|Storage buffer||whole serum|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Suggested positive control: HL-60 cell extracts, antigen standard for CORO1A (transient overexpression lysate).
Highly conserved coronin family of proteins/actin regulators from WD-repeat superfamily were originally identified in Dictyostelium, wherein, coronin deletions were observed to affect actin-dependent processes, such as phagocytosis, cell motility, and cytokinesis. Coronin-1A (Coro1A) belongs to Type I coronins, almost exclusively expressed in hematopoietic lineages, and plays a key role during T lymphocyte activation, migration, survival, and calcium signaling. Moreover, Coronin-1A has been documented to be dispensable for actin-dependent functions in macrophages. Coro1a-deficient mice have reduced peripheral T cells due to both an impaired survival of naive cells and migrational defects. Both CD4+ and CD8+ T cell subsets are decreased in the periphery of Coro1a-/- mice while thymic cellularity and subpopulations are similar to those observed in wild-type mice. A nonsense mutation in Coro1a has been shown to protect against systemic lupus in a mouse model. Moreover, it has shown that Coro1a is mutated in a thymic egress-deficient mouse strain, but also in a patient with severe combined immunodeficiency (SCID).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CLABP; Clipin-A; CLIPINA; coronin, actin binding protein, 1A; coronin-1; Coronin-1A; Coronin-like protein A; Coronin-like protein p57; FLJ41407; HCORO1; MGC117380; p57; TACO; Tryptophan aspartate-containing coat protein
Gene Aliases: CLABP; CLIPINA; CORO1; CORO1A; HCORO1; IMD8; p57; TACO
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