|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Immunoprecipitation (IP)||1 µg|
|Western Blot (WB)||1:1000|
|Western Blot (WB)||See 3 publications below|
|Tested Species reactivity||Bovine, Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Bovine Cyclin A fusion protein|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
MA1-154 detects Cyclin A in mammalian samples and has been successfully used in Immunohistochemistry (Frozen and Paraffin), Immunofluorescence, Immunoprecipitation and Western Blot procedures. Immunoprecipitation and Western Blot analysis with MA1-154 show the accumulation of a prominent band at ~50 kDa in camptothecin and hydroxyurea treated cells. In Immunofluorescence applications, MA1-154 shows nuclear translocation of Cyclin A in G2/M arrested cells as a result of camptothecin treatment.
The protein encoded by the CCNA2 gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, cyclin A2 is expressed in all tissues tested. Cyclin A binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CCN1; CCNA; CCNA2; Cyclin A; Cyclin A2; Cyclin A2 (Cyclin A); Cyclin-A; Cyclin-A2
Gene Aliases: AA408589; BOS_6308; Ccn-1; CCN1; CCNA; CCNA2; Cyca; CycA2
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