|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:500|
|Western Blot (WB)||1:1000|
|Tested Species reactivity||Human, Mouse, Non-human primate, Xenopus|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Xenopus laevis cyclin B2|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
MA1-156 detects Cyclin B2 in mammalian samples. MA1-156 has been successfully used in Immunofluorescence, Immunoprecipitation, IHC (P) and Western Blot procedures. Immunoprecipitation and Western Blot analysis with MA1-156 show the accumulation of a prominent band at ~51 kDa in camptothecin and hydroxyurea treated cells. MA1-156 also detects additional unknown band at ~80 kDa. In Immunofluorescence applications, MA1-156 shows cyclin B2 staining consistent with the Golgi region, whereas MA1-155 shows cyclin B1 co-localization with microtubules.
MA1-156 reacts with Cyclin B2 from Xenopus laevis and mammalian sources. In Western blot applications, X29.2 also cross reacts with Cyclin B1.
Cyclins bind to and regulate the activity of the Cyclin Dependent Protein Kinases (CDKs). The protein encoded by the CCNB2 gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Cyclin B2 steadily accumulates during G2 phase, followed by abrupt APC dependent destruction at the end of mitosis. Destruction of Cyclin B2 is required for cell cycle progression, as destruction resistant mutants cause mitotic arrest. Cyclins B1 and B2 differ in their subcellular localization. Cyclin B1 co-localizes with microtubules, whereas cyclin B2 is primarily associated with the Golgi region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C-B2; CCNB2; Cyclin B2; G2/mitotic-specific cyclin B2; G2/Mitotic-Specific Cyclin-B2; HsT17299
Gene Aliases: CCNB2; CycB2; cyclinb2; HsT17299
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