|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||10µg/mg protein lysate|
|Western Blot (WB)||2 µg/mL|
|Tested Species reactivity||Dog, Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide corresponding to aa 290-303 (Cys-QGPNGSQNSSYSQS) from the C-terminal of human cyclin C|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
PA5-16227 targets Cyclin C in immunofluorescence, immunoprecipitation, and Western blot applications and shows reactivity with Canine, Human, mouse, and Rat samples.
The PA5-16227 immunogen is a synthetic peptide corresponding to aa 290-303 (Cys-QGPNGSQNSSYSQS) from the C-terminal of human cyclin C.
Cyclin C was originally identified by a genetic screen for human and Drosophila cDNAs that complement a triple knock-out of the CLN genes in Saccharomyces cerevisiae. Cyclin C is a nuclear protein present in a multiprotein complex. It interacts both in vitro and in vivo with cdk8, a novel protein-kinase of the cdk family. It is suggested that cyclin C and cdk8 control RNA polymerase II function. Human cdk8-cyclin C might be functionally associated with the mammalian transcription apparatus, perhaps involved in relaying growth-regulatory signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cyclin-C; dJ199J3.4 (cyclin C); hSRB11; SRB11 homolog
Gene Aliases: AI451004; AU020987; CCNC; CG1C; CycC
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