|Western Blot (WB)||1:1000-1:10,000|
|Tested Species reactivity||Luciferase|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified recombinant Cypridina luciferase expressed in E. coli.|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
Western blot analysis of PA1-182 detects a ~62 kDa protein in the supernatant of Cypridina luciferase-secreting cells.
PA1-182 can be used for activity-independent verification of Cypridina luciferase expression.
The Cypridina luciferase is a 62kDa protein from the marine ostracod, Cypridina noctiluca. The bioluminescent enzyme is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output generated by the luciferase reaction can be correlated to the amount of Cypridina luciferase protein produced and used to determine the activity of the promoter driving Cypridina expression. Cypridina luciferase can be measured individually or in multiplex assays, such as the Cypridina-Firefly Luciferase Dual Assay Kit (Product # 16183). The Cypridina luciferase has been codon optimized for enhanced performance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cypridina luciferase; Cypridina noctiluca luciferase
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.