|ELISA (ELISA)||Assay Dependent|
|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||1:100 - 1:500|
|Immunoprecipitation (IP)||2 µg/ml|
|Immunocytochemistry (ICC)||See 2 publications below|
|Tested Species reactivity||Bovine, Dog, Horse, Human, Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Equine cytochrome c|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-11823 targets Cytochrome c in ELISA, FACS, immunofluorescence and immunoprecipitation applications and shows reactivity with Bovine, Canine, Equine, Mouse and Human samples. MA5-11823 is not suitable for Western blotting applications.
This antibody was orginally validated as part of a Thermo Scientific Cellomics High Content Screening Kit. The antibody sold separately may have slightly different performance and may need to be further optimized for the best results.
Cytochrome c is an electron transporting protein that resides within the inter-membrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytocrome c has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome c is rapidly released from the mitochondria into the cytosol, an event which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome c functions in the activation of caspase-3, and ICE family molecule that is a key effector of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cytochrome c; Cytochrome c, somatic; cytochrome c-like protein
Gene Aliases: BOS_4320; CYC; CYCS; cytc; HCS; THC4
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