|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Western Blot (WB)||2 µg/ml|
|Immunohistochemistry (IHC)||See 11 publications below|
|Blocking Assay (BLOCK)||See 2 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||A synthetic peptide from the C-terminus of human cytokeratin 16|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-13730 targets Cytokeratin 16 in immunofluorescence and immunohistochemistry (paraffin) applications and shows reactivity with Human samples. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
The MA5-13730 immunogen is a synthetic peptide from the C-terminus of human cytokeratin 16.
Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 16 is absent in normal breast tissue and in noninvasive breast carcinomas. Only 10% of the invasive breast carcinomas show diffuse or focal positivity. Reportedly, a relatively high concordance was found between the carcinomas immunostaining with the basal cell and the hyperproliferation-related keratins, but not between these markers and the proliferation marker Ki-67. This supports the conclusion that basal cells in breast cancer may show extensive proliferation, and that absence of Ki-67 staining does not mean that (tumor) cells are not proliferating.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CK-16; cytokeratin 16; Cytokeratin-16; focal non-epidermolytic palmoplantar keratoderma; K16; keratin; keratin 16, type I; Keratin, type I cytoskeletal 16; Keratin-16; type I cytoskeletal 16
Gene Aliases: CK16; FNEPPK; K16; K1CP; KRT16; KRT16A; NEPPK; PC1
UniProt ID: (Human) P08779
Entrez Gene ID: (Human) 3868
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