|Flow Cytometry (Flow)||1:100 - 1:200|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
|Western Blot (WB)||1:100 - 1:1000|
|Tested Species reactivity||Dog, Cat, Hamster, Human, Mouse, Pig, Rabbit, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Cytokeratins from human lung cancer cell line MR21.|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
MA1-06314 detects cytokeratin 5 and 8 in human, mouse, rat, rabbit, hamster, canine, swine, and feline samples.
MA1-06314 has sucessfully been used in Western blotting, flow cytometry, immunocytochemistry and immunohistochemistry applications.
The MA1-06314 immunogens are cytokeratins from a human lung cancer cell line (MR21).
Store at 4°C or in small aliquots at -20°C.
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between ~40 kDa and ~68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 58 kDa cytokeratin; CK-5; CK-8; CYK8; cytokeratin 5; cytokeratin 8; Cytokeratin endo A; Cytokeratin-5; Cytokeratin-8; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K5; K8; keratin 5; keratin 5 (epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); keratin 5, type II; keratin 5-like; keratin 8; keratin 8, type II; keratin complex 2, basic, gene 5; keratin complex 2, basic, gene 8; Keratin type II cytoskeletal 5; Keratin type II cytoskeletal 8; Keratin, type II cytoskeletal 5; keratin, type II cytoskeletal 5-like protein; Keratin, type II cytoskeletal 8; keratin, type II cytoskeletal 8-like protein; Keratin-5; Keratin-8; KRT5; KRT8; tuftelin interacting protein 8; Type-II keratin Kb5; Type-II keratin Kb8
Gene Aliases: 3300001P10Rik; AA960620; AL022697; AU019895; AW146334; CARD2; CK-8; CK5; CK8; CYK8; CYKER; DDD; DDD1; EBS2; EndoA; K2C8; K5; K8; KO; Krt-2.8; Krt2-5; Krt2-8; KRT5; KRT5A; KRT8; Sak57; Tfip8
Entrez Gene ID: (Dog) 100687760, (Human) 3852, (Dog) 486513, (Human) 3856, (Rabbit) 100348891, (Chinese hamster) 100754346, (Rat) 369017, (Mouse) 110308, (Mouse) 16691, (Chinese hamster) 100759488, (Rat) 25626, (Rabbit) 100352658
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