|Flow Cytometry (Flow)||1:100 - 1:200|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
|Western Blot (WB)||1:100 - 1:1000|
|Immunohistochemistry (IHC)||See 34 publications below|
|Immunocytochemistry (ICC)||See 3 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Fish , Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Cytoskeletal preparation of OTN-11 ovarian carcinoma cell line.|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
MA1-06316 detects Cytokeratin 7 in human samples.
MA1-06316 has sucessfully been used in Western blotting, immunocytochemistry, flow cytometry, and immunohistochemistry. Pretreatment is required for paraffin sections; 0.1% pronase/0.1% trypsine in PBS or 0.5% pepsine in HCL or microwave pretreatment suggested.
The MA1-06316 immunogen is a cytoskeletal preparation of OTN-11 ovarian carcinoma cell line.
Store at 4°C, or in small aliquots at -20°C.
Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between ~40 kDa and ~68 kDa and isoelectric pH between 4.9-7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CK-7; cytokeratin 7; Cytokeratin-7; K7; Keratin; keratin 7, type II; keratin, 55K type II cytoskeletal; keratin, simple epithelial type I, K7; Keratin, type II cytoskeletal 7; Keratin-7; Sarcolectin; type II cytoskeletal 7; type II mesothelial keratin K7; Type-II keratin Kb7
Gene Aliases: CK7; K2C7; K7; KRT7; SCL
UniProt ID: (Human) P08729
Entrez Gene ID: (Human) 3855
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