|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 741 and 907 of Human DAP5|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-27489 targets DAP5 in WB applications and shows reactivity with Human and mouse samples.
The PA5-27489 immunogen is recombinant fragment corresponding to a region within amino acids 741 and 907 of Human DAP5.
Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: AAG1; aging-associated protein 1; DAP-5; DAP5; Death-associated protein 5; eIF-4-gamma 2; eIF-4G 2; eIF4G 2; EIF4G2; Eukaryotic translation initiation factor 4 gamma 2; eukaryotic translation initiation factor 4 gamma, 2; eukaryotic translation initiation factor 4G-like 1; NAT1; Novel APOBEC-1 target 1; P97; Translation repressor NAT1
Gene Aliases: AA589388; AAG1; DAP-5; DAP5; E130105L11Rik; EIF4G2; NAT1; Natm1; OK/SW-cl.75; P97
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