|Immunofluorescence (IF)||20 µg/ml|
|Immunohistochemistry (IHC)||20 ug/ml|
|Western Blot (WB)||1-2 ug/ml|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 14 amino acid peptide near the amino terminus of human DCLK3.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
A suggested positive control is K562 cell lysate.
PA5-20911 can be used with blocking peptide PEP-1025.
DCLK3 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK3 mRNA, like that of the homologous DCLK1 and DCLK3, is highly expressed in adult brain, but only DCLK3 transcripts are present in liver and kidney, suggesting that DCLK3 may play other roles than in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a protein that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CLICK-I and II-related; Click-I, -II related; CLICK-I,II-related; CLr; doublecortin and CaM kinase-like 3; Doublecortin domain-containing protein 3C; Doublecortin-like and CAM kinase-like 3; Doublecortin-like kinase 3; hCG_16695, CLR, DCAMKL3, DCDC3C, DCK3; Serine/threonine-protein kinase DCLK3
Gene Aliases: BC056929; C730036H08; CLR; DCAMKL3; DCDC3C; DCK3; DCLK3; KIAA1765
Molecular Function: non-receptor serine/threonine protein kinase
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