|Flow Cytometry (Flow)||1:25-1:50|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Blocking Assay (BLOCK)||See 1 publications below|
|Tested Species reactivity||Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Dectin-2 (extracellular domain) Fc Fusion protein.|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
Dectin-2 is a C-type lectin expressed by dendritic cells, macrophages and inflammatory monocytes. It is a type II membrane receptor, with a short cytoplasmic tail, and a C-terminal C-type lectin domain. Dectin-2 is a receptor with specificity for high mannose structures and mediates cytokine production from DC and other antigen presenting cells. Though, Dectin-2 has no obvious signaling motifs attached to CP tail, reports indicate that Dectin-2 employs FcRg for internalization and to activate NF-kappaB pathway for cytokine production. Though it shares only 20-25% of homology with Dectin-1, it functions similarly as pattern recognition receptor (PRR) for fungal infections, to activate both innate (Syk-CARD9 innate signaling pathway) and adaptive immune responses (by activation of DC) to fungal infections. Thus Dectin-2 is a connector molecule of both innate and adaptive immunity functioning at DC level.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C-type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 10; C-type lectin domain family 6 member A; C-type lectin superfamily member 10; Clec6a, Clecsf10, Nkcl,C-type (calcium dependent, carbohydrate recognition domain) lectin, superfamily member 10; DC-associated C-type lectin 2; dectin-2; Dendritic cell-associated C-type lectin 2; natural killer complex lectin
Gene Aliases: Clec4n; Clec6a; Clecsf10; Dectin2; Nkcl
UniProt ID: (Mouse) Q9JKF4
Entrez Gene ID: (Mouse) 56620
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