|Western Blot (WB)||1:100-1:2000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 144-129 of the DEDD2 protein.|
|Storage buffer||whole serum|
|Contains||0.02% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Suggested positive control: antigen standard for DEDD2 (transient overexpression lysate).
Apoptotic signals are often triggered by cell surface death receptors through protein-protein interactions mediated by conserved domains such as the death effector domain. A novel death effector domain (DED)-containing protein, DEDD2, has been recently identified. Over-expression of DEDD2 in transfected cells induces moderate apoptosis and results in substantial sensitization to apoptosis induced by Fas, TRAIL, and FADD. More recently, it has been shown that DEDD2 can bind caspase-8 and -10 in addition to FLIP but not FADD. Like the related protein DEDD, DEDD2 translocates from the cytosol to the nucleus upon induction of apoptosis, and it has been suggested that DEDD2 may target caspase-8 to the nucleus and that DEDD2 thus plays a critical role in death receptor-induced apoptosis. At least two alternatively spliced transcript variants encoding distinct isoforms have been found for DEDD2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: death effector domain-containing DNA binding protein 2; DED-containing protein FLAME-3; DNA-binding death effector domain-containing protein 2; FADD-like anti-apoptotic molecule 3; FLAME-3; PSEC0004
Gene Aliases: DEDD2; FLAME-3; FLAME3; PSEC0004
UniProt ID: (Human) Q8WXF8
Entrez Gene ID: (Human) 162989
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