|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:500|
|Western Blot (WB)||0.5-2 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptides corresponding to residues 199-212 and 226-239 of human Smac.|
|Contains||0.02% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Suggested positive control: Hela whole cell extract, HeLa cell lysate.
Recently, three different laboratories have identified a molecule, Smac/DIABLO, which binds with inhibitor of apoptosis proteins (IAPs) and neutralizes IP inhibition of effector caspases. Multiple IAP molecules, including c-IAP1, c-IAP2, XIAP, and survivin are able to bind Smac. Smac/DIABLO is a mitochondrial protein, which is released into cytosol during apoptosis. The function of Smac is similar to that of Drosophila apoptosis activating proteins Reaper, Hid and Grim, which have been proposed to function by neutralizing the activity of IAPs. Smac promotes caspase-3 and caspase-9 activation by removing inhibitors rather than directly acting on Apaf-1, procaspases, and cytochrome c. The full-length cDNA of Smac encodes an open reading frame of 239 amino acids, predicting a protein with a molecular weight of 27 kDa. However, the mature protein purified from HeLa cells is only about 25 kDa (3).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DIABLO; Diablo homolog, mitochondrial; diablo, IAP-binding mitochondrial protein; diablo-like protein; Direct IAP-binding protein with low pI; Second mitochondria-derived activator of caspase; Smac
Gene Aliases: DFNA64; DIABLO; SMAC
UniProt ID: (Human) Q9HAV6
Entrez Gene ID: (Human) 56616
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.