|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Western Blot (WB)||1:500|
|Western Blot (WB)||See 3 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C-D(435) T I I D V V G A P L T R N S R(450) of mouse DMAP1.|
|Purification||Ammonium sulfate precipitation|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-886 detects DNA methyltransferase 1 associated protein (DMAP1) from human and mouse cells.
PA1-886 has been successfully used in Western blot and Immunohistochemistry (paraffin) procedures. By Western blot, this antibody detects an ~52 kDa band which corresponds to DMAP1 from HeLa cell nuclear extracts.
PA1-886 immunizing peptide corresponds to amino acid residues 435-450 from mouse DMAP1. This sequence is completely conserved in human DMAP1. PA1-886 immunizing peptide (Cat. # PEP-118) is available for use in neutralization and control experiments.
DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X-chromosome inactivation. DNA methyltransferase 1 associated protein (DMAP1) has been shown to interact directly with the N-terminus of DNA Methyltransferase 1 (Dnmt1) throughout DNA replication during S phase. DMAP1 has also been identified as a critical gene product in myotonic dystrophy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DNA Methyltransferase 1 Associated Protein; DNA methyltransferase 1-associated protein 1; DNMAP1; DNMT1 associated protein 1; DNMT1 associated protein-1; DNMT1-associated protein 1; MAT1-mediated transcriptional repressor
Gene Aliases: 1500016M21Rik; DMAP1; Dmtap1; DNMAP1; DNMTAP1; EAF2; KIAA1425; MEAF2; mKIAA1425; Mmtr; SWC4
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