|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||Assay dependent|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Full-length recombinant human DNA Ligase I protein|
|Storage buffer||PBS, pH 7.2|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA1-23189 detects DNA ligase I in Human and Mouse samples.
MA1-23189 has been successfully used in Immunoprecipitation and Western Blot procedures.
MA1-23189 immunogen corresponds to Full-length recombinant human DNA Ligase I protein.
There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA ligase I migrates as a 125kDa phosphoprotein and is implicated in joining Okazaki fragments during DNA synthesis. It also has a role in the base and nucleotide excision repair pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DNA ligase 1; DNA ligase I; ligase I, DNA, ATP-dependent; MGC117397; MGC130025; Polydeoxyribonucleotide synthase [ATP] 1
Gene Aliases: AL033288; Lig-1; LIG1; LigI
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.