|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Gel Shift (GS)||1mg/mL|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||2-4 µg/ml|
|Immunoprecipitation (IP)||2 µg/mg protein lysate|
|Western Blot (WB)||1:1000|
|Immunoprecipitation (IP)||See 6 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Western Blot (WB)||See 55 publications below|
|Immunohistochemistry (IHC)||See 4 publications below|
|Immunocytochemistry (ICC)||See 6 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|ChIP assay (ChIP)||See 1 publications below|
|Gel Shift (GS)||See 1 publications below|
|Tested Species reactivity||Chicken, Hamster, Human, Mouse, Xenopus|
|Published species reactivity||Pig , Rat , Human , Mouse|
|Host / Isotype||Mouse / IgG1/IgG2a|
|Clone||18-2 + 25-4 + 42-psc|
|Immunogen||Human DNA-dependent Protein Kinase purified from HeLa cells|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-13404 targets DNA-PKcs in gel shift, immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation, and western blot applications and shows reactivity with Chicken, Hamster, Human, mouse, and Xenopus laevis samples.
The MA5-13404 immunogen is human DNA-dependent Protein Kinase purified from HeLa cells.
DNA-dependent Protein Kinase (DNA-PKcs) phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. In vivo, it is involved in repairing double strand DNA breaks
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: complementing 1; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNPK1; doxorubicin nephropathy; hyper-radiosensitivity of murine scid mutation; hyper-radiosensitivity of murine scid mutation, complementing 1; p460; protein kinase, DNA activated, catalytic polypeptide; protein kinase, DNA-activated, catalytic polypeptide; severe combined immunodeficiency
Gene Aliases: AI326420; AU019811; DNA-PKCS; DNAPDcs; DNAPK; DNPK1; DOXNPH; dxnph; HYRC; HYRC1; I79_017613; IMD26; p350; p460; PRKDC; prkdc-A; scid; slip; XRCC7
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