|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-100|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
|Western Blot (WB)||See 25 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|Immunoprecipitation (IP)||See 3 publications below|
|ChIP assay (ChIP)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Gel Shift (GS)||See 1 publications below|
|Tested Species reactivity||Human, Rat|
|Published species reactivity||Human Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Human DNA-dependent Protein Kinase purified from HeLa cells|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-13235 targets DNA-PKcs in IF, IHC (P), IP, and WB applications and shows reactivity with Human and Rat samples.
The MA5-13235 immunogen is human DNA-dependent Protein Kinase purified from HeLa cells.
DNA-dependent Protein Kinase (DNA-PKcs) phosphorylates transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. In vivo, it is involved in repairing double strand DNA breaks.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: complementing 1; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNPK1; hyper-radiosensitivity of murine scid mutation; hyper-radiosensitivity of murine scid mutation, complementing 1; p460
Gene Aliases: DNA-PKcs; DNAPK; DNPK1; HYRC; HYRC1; IMD26; p350; PRKDC; XRCC7
UniProt ID: (Human) P78527
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