|Immunohistochemistry (IHC)||5 µg/ml|
|Western Blot (WB)||0.5 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids 347 to 360 of human DNase II precursor (2-4).|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
In Western blot applications, this antibody detects a band at ~40kDa, which represents the pro-enzyme of DNase II. A suggested positive control is human spleen tissue lysate.
PA5-19900 can be used with blocking peptide PEP-0026.
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus. DNase II causes both chromatin condensation and DNA fragmentation. The genes encoding human, porcine, and murine DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (alpha) and 12 kDa (beta) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation. DNase II is ubiquitously expressed in human tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Acid DNase; Deoxyribonuclease II alpha; Deoxyribonuclease-2-alpha; DNase II alpha; DNase II, lysosomal; DNASE2A, DNL, DNL2; Lysosomal DNase II; R31240_2
Gene Aliases: DNASE2; DNASE2A; DNL; DNL2
UniProt ID: (Human) O00115
Entrez Gene ID: (Human) 1777
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