|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide of human DOG1 protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is gastrointestinal stromal tumor.
DOG-1, the novel marker, is expressed ubiquitously in gastrointestinal stromal tumors irrespective of kit or PDGFR alpha mutation status.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: anoctamin 1, calcium activated chloride channel; Anoctamin-1; Ca2+-activated Cl- channel; calcium activated chloride channel; Discovered on gastrointestinal stromal tumors protein 1; DOG1; oral cancer overexpressed 2; Oral cancer overexpressed protein 2; ORAOV2; TAOS2; TMEM16A; Transmembrane protein 16A; transmembrane protein 16A (eight membrane-spanning domains); transmembrane protein 16A eight membrane-spanning domains; Tumor-amplified and overexpressed sequence 2
Gene Aliases: ANO1; DOG1; ORAOV2; TAOS2; TMEM16A
UniProt ID: (Human) Q5XXA6
Entrez Gene ID: (Human) 55107
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