|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||10 ug/ml|
|Western Blot (WB)||1 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to amino acids near the carboxy terminus of human DOK1.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
In Western blot applications, this antibody detects a band at ~62kDa. A suggested positive control is Jurkat cell lysate.
PA5-19860 can be used with blocking peptide PEP-0257.
Signals from most growth factors and cytokines are transduced by receptor tyrosine kinases or non-receptor tyrosine kinases. Activated tyrosine kinases phosphorylate their substrates, which mediate the cellular response to extracellular stimuli. A long-sought major substrate termed p62dok (downstream of tyrosine kinase) for many tyrosine kinases including c-kit, v-abl, v-Fps, v-Src, v-Fms, and activated EGF, PDGF, IGF, VEGF and insulin receptors was identified recently from human and mouse by several laboratories. Upon phosphorylation, p62dok forms a complex with the ras GTPase-activating protein (RasGAP). p62dok represents a new family with very recently identified p56dok.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Docking protein 1; docking protein 1, 62kDa (downstream of tyrosine kinase 1); Downstream of tyrosine kinase 1; p62(dok); pp62; telomerase protein 1; telomerase protein component 1; TLP1, TP1, TROVE1, VAULT2, p240; TROVE domain family, member 1
Gene Aliases: DOK1; P62DOK; pp62
UniProt ID: (Human) Q99704
Entrez Gene ID: (Human) 1796
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.