|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 9 and 277 of Human DOK1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-27464 targets DOK1 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-27464 immunogen is recombinant fragment corresponding to a region within amino acids 9 and 277 of Human DOK1.
Docking protein 1 is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. Docking protein 1 contains a putative pleckstrin homology domain at the amino terminus and ten PXXP SH3 recognition motifs. Docking protein 2 binds p120 (RasGAP) from CML cells. It has been postulated to play a role in mitogenic signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Docking protein 1; docking protein 1 (downstream of tyrosine kinase 1); docking protein 1, 62kDa (downstream of tyrosine kinase 1); Downstream of tyrosine kinase 1; p62(dok); P62DOK; pp62
Gene Aliases: DOK1; P62DOK; pp62
UniProt ID: (Human) Q99704
Entrez Gene ID: (Human) 1796
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.