|Western Blot (WB)||0.3-1 ug/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide sequence (SYLQSPITTSPSC) corresponding to the C-terminus amino acids of DUSP1|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This antibody is predicted to react with bovine and canine based on sequence homology.
The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 3CH134/CL100 PTPase; CL 100; CL100; dual specificity phosphatase 1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUSP1; HVH1; MAP kinase phosphatase 1; MAP kinase phosphatase-1; mitogen-activated protein (MAP) kinase phosphatase-1; Mitogen-activated protein kinase phosphatase 1; mitogen-activated protein kinase phosphatase-1; MKP-1; MKP1; oxidative stress-inducible protein tyrosine phosphatase; protein tyrosine phosphatase non-receptor type 16; protein tyrosine phosphatase, non-receptor type 16; Protein-tyrosine phosphatase 3CH134; Protein-tyrosine phosphatase CL100; Protein-tyrosine phosphatase ERP; Protein-tyrosine phosphatase non-receptor type 16; PTPN10; serine/threonine specific protein phosphatase
Gene Aliases: 3CH134; CL100; DUSP1; erp; HVH1; MKP-1; MKP1; PTPN10; Ptpn16; U19515; VH1
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