|Immunohistochemistry (Frozen) (IHC (F))||1:1000|
|Western Blot (WB)||1:1000|
|Tested Species reactivity||Human, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acid residues from the extracellular loop 2 region of human SLC6A3 conjugated to KLH|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This antibody is specific for the ~88 kDa DAT protein in Western blots of SDS-solubilized human striatal samples and in IHC applications with formaldehyde-fixed human and monkey brain sections.
Store at -20°C short term, 80°C long term.
The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. DAT antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson and quote;s disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). Levels of DAT protein expression are altered by chronic drug administration (Wilson et al., 1996).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DA transporter; DAT; dopamine transporter 1; SLC6A3; Sodium-dependent dopamine transporter; solute carrier family 6 (neurotransmitter transporter), member 3; solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; Solute carrier family 6 member 3
Gene Aliases: DAT; DAT1; PKDYS; SLC6A3
UniProt ID: (Human) Q01959
Entrez Gene ID: (Human) 6531
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.