|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||2 µg/ml|
|Western Blot (WB)||See 10 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat , Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide corresponding to resdiues C K(633) E K A S E T E E N G S D S F(647) of rat Dyn1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-660 detects dynamin I (Dyn1) from rat tissues.
PA1-660 has been successfully used in Western blot and immunocytochemical procedures. By Western blot, this antibody detects an ~100 kDa protein representing Dyn1 from rat whole brain extract.
PA1-660 immunizing peptide corresponds to amino acid residues 633-647 from rat Dyn1. This sequence is completely conserved between rat, mouse, and human Dyn1. PA1-660 immunizing peptide (Cat. # PEP-042) is available for use in neutralization and control experiments.
The dynamins are a family of 100 kDa GTPases transcribed from at least three separate genes. At least four mRNA splice variants for each dynamin have been described. Dynamins contain several conserved regions including the conserved, amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a coiled-coil region located in front of a proline-rich domain (PRD). The PRD is thought to mediate interactions between dynamin and numerous other cellular proteins. Dynamin I is expressed exclusively in neurons, dynamin II is ubiquitously expressed, and dynamin III is thought to be restricted to expression in the brain, testis, heart, and lung. The dynamins participate in the cellular process of clathrin-mediated and fluid-phase endocytosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: B-dynamin; D100; Dyn1; Dynamin, brain; Dynamin-1
Gene Aliases: AI838169; DNM; DNM1; EIEE31; Ftfl; Kiaa4093; mKIAA4093
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