|Western Blot (WB)||1:1,000|
|Western Blot (WB)||See 3 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: C P(623) D K S F T E N D E N G Q A E N F(639)|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-662 detects dynamin III (Dyn3) from rat, mouse, and human tissues and cells.
PA1-662 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~100 kDa protein representing Dyn3 from HeLa cell lysate.
PA1-662 immunizing peptide corresponds to amino acid residues 623-639 from rat Dyn3. PA1-662 immunizing peptide (Cat. # PEP-057) is available for use in neutralization and control experiments.
The dynamins are a family of 100 kDa GTPases transcribed from at least three separate genes. At least four mRNA splice variants for each dynamin have been described. Dynamins contain several conserved regions including the conserved, amino-terminal GTPase domain, a centrally located membrane-binding plekstrin homology domain (PHD), and a coiled-coil region located in front of a proline-rich domain (PRD). The PRD is thought to mediate interactions between dynamin and numerous other cellular proteins. Dynamin I is expressed exclusively in neurons, dynamin II is ubiquitously expressed, and dynamin III is thought to be restricted to expression in the brain, testis, heart, and lung. The dynamins participate in the cellular process of clathrin-mediated and fluid-phase endocytosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Dyn3; Dyna III; dynamin family member; Dynamin III; Dynamin, testicular; Dynamin-3; T-dynamin; testicular dynamin
Gene Aliases: 9630020E24Rik; AW061159; B230343F03Rik; C530045C17; DNM3; Dyn3; Dyna III; dynamin-2; KIAA0820; mKIAA0820
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.