|Immunocytochemistry (ICC)||Assay dependent|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1 µg/ml|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 3 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Pig , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide corresponding to C-terminal of human Dystrophin.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C, do not freeze|
PA1-21011 detects Dystrophin in Human, Mouse and Rat samples.
PA1-21011 has been successfully used in Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin) immunocytochemistry, immunofluorescence, and Western Blot procedures. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. Positive control of skeletal muscle suggested. Molecular weight of 427 kDa is expected.
PA1-21011 immunogen corresponds to a synthetic peptide corresponding to C-terminal of human Dystrophin.
Dystrophin is a member of the spectrin/alpha-actinin family of actin-binding, triple helix rod-containing proteins. It is absent or greatly reduced in individuals with the X-linked recessive Duchenne's muscular dystrophy disorder, as well as in mice with the mdx (murine muscular dystrophy) mutation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: apodystrophin-3; apodystrophin-I; BMD; CMD3B; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; Dystrophin; dystrophin Dp40; dystrophin Dp71a; dystrophin Dp71ab; dystrophin Dp71b; dystrophin, muscular dystrophy; GS1-19O24.1; X-linked muscular dystrophy
Gene Aliases: BMD; CMD3B; DMD; DNADMD1; Dp427; Dp71; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; DXSmh7; DXSmh9; dys; mdx; MRX85; pke
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