|Gel Shift (GS)||1mg/mL|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||2µg/mg protein lysate|
|Western Blot (WB)||1-2 µg/ml|
|Immunohistochemistry (IHC)||See 3 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 3 publications below|
|Western Blot (WB)||See 4 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2a, kappa|
|Immunogen||Recombinant human E2F-1 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-14344 targets E2F-1 Transcription Factor in gel shift, immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation, and Western blot applications and shows reactivity with Human and mouse samples.
The MA5-14344 immunogen is recombinant human E2F-1 protein.
E2F's are DNA-binding proteins that associate with negative regulators, such as the retinoblastoma p107 protein, resulting in an altered rate of gene transcription. E2F-1 also requires DP-1 for efficient DNA-binding and transcription modification. E2F1 is proposed to be involved in several cellular processes that range from tumor suppression, cell cycle progression, and oncogenesis. E2F-1 overexpression can also drive cells into apoptosis. These observations place E2F among a group of apoptosis inducers that includes; c-Myc, Adenovirus E1A, and HPV E7 protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: E2F-1; PBR3; PRB-binding protein E2F-1; RBAP-1; RBBP-3; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; Transcription factor E2F1
Gene Aliases: E2F-1; E2F1; mKIAA4009; RBAP1; RBBP3; RBP3
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.