|Flow Cytometry (Flow)||1-3 µg/10^6 cells|
|Western Blot (WB)||0.1-3 µg/ml|
|Tested Species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b, Kappa|
|Immunogen||full length recombinant mouse EBI3|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
EBI3 (Epstein-Barr virus Induced-3) is a secreted glycoprotein of the hematopoietin receptor family. It plays a critical regulatory role in the induction of Th2-type immune responses and the development of Th2-mediated tissue inflammation in vivo, which may be mediated through the control of iNKT cell function. EBI3 dimerizes with p28 and p35 subunits of IL-12 to form new proteins IL-27 and IL-35, respectively. IL-27 is an early product of activated antigen presenting cell that is produced upon TLR ligation. It negatively regulates Th17 cell differentiation. EBI3 is widely expressed and its expression in dendritic cell is transcriptionally regulated by TLR signaling via MyD88 and NF-kappaB during innate immune responses preceding cytokine driven Th cell development. EBI3 signal inhibits delayed-type hypersensitivity responses by suppressing IL-17 production and inducing IL-10 hyperproduction. EBI3 may play a novel role in controlling tumor metastasis via lung CD8+ T cells, and its deficiency is associated with a diminished production of Th2 cytokines which are known to regulate allergic airway inflammation in asthma. Targeted deletion of EBI3 protects mice from lung metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cytokine receptor; Epstein-Barr virus induced gene 3; epstein-Barr virus-induced gene 3 protein; Epstein-Barr virus-induced gene 3 protein homolog; IL-27 subunit beta; IL-27B; IL27 subunit; IL27B; IL35 subunit; Interleukin-27 subunit beta
Gene Aliases: EBI-3; Ebi3; IL-27; Il27b
UniProt ID: (Mouse) O35228
Entrez Gene ID: (Mouse) 50498
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