|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||10 µg/ml|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||See 3 publications below|
|Immunohistochemistry (IHC)||See 11 publications below|
|Immunocytochemistry (ICC)||See 4 publications below|
|Western Blot (WB)||See 7 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Dog , Rat , Mouse , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Bacterially expressed, His-tagged human EBP50.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-090 detects ezrin-radixin-moesin (ERM) binding phosphoprotein of 50 kDa (EBP50) from mouse tissues, human T84 and Calu3 cells as well as recombinant human protein.
PA1-090 has been successfully used in Western blot, Immunohistochemistry, immunoflourescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~50 kDa protein representing EBP50 from T84 cell extract. Immunohistochemical staining of EBP50 in mouse airway results in staining of the epithelium.
The PA1-090 immunogen is recombinant human His-tagged EBP50 obtained from bacteria. All His-tag specific antibody has been removed. This product was epitope affinity purified using a synthetic peptide to residues C R(286) S A S S D T S E E L N S Q D S P(302) of human EBP50
EBP50 [ERM (ezrin-radixin-moesin) binding phosphoprotein of 50 kDa] is a PDZ containing protein that is involved in the linkage of integral membrane proteins to the cytoskeleton. EBP50 contains two tandem PDZ domains followed by a carboxy-terminal sequence that binds to members of the ERM family of membrane-cytoskeleton adaptors. The PDZ domains within EBP50 bind to the carboxy termini of such target proteins as beta2 adrenergic receptor, platelet-derived growth factor receptor (PDGFR), and the cystic fibrosis conductance regulator (CFTR). The PDZ domains are also believed to be involved in the oligomerization of EBP50 to form multiprotein complexes which helps to facilitate the formation of functional signalling complexes.
EBP50 has been shown to link integral membrane proteins to the cytoskeleton by binding to members of the ERM family of proteins, which bind to F-actin. Through these interactions, EBP50 is implicated in the localization of interactive groups of proteins into subcellular domains and in the regulation of activity of those interacting proteins. Immunohistochemical studies have shown that EBP50 is found exclusively at the apical membranes of epithelial cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: EBP50; Ezrin-Radixin-Moesin Binding Phosphoprotein 50; ezrin-radixin-moesin binding phosphoprotein-50; Ezrin-radixin-moesin-binding phosphoprotein 50; Na(+)/H(+) exchange regulatory cofactor NHE-RF; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na+/H+ exchange regulatory co-factor; NHERF; NHERF-1; Regulatory cofactor of Na(+)/H(+) exchanger; So; Sodium-hydrogen exchanger regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatory factor 1; Solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
Gene Aliases: EBP-50; EBP50; NHE-RF; NHERF; NHERF-1; NHERF1; NPHLOP2; SLC9A3R1
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