|Western Blot (WB)||2-4 µg/ml|
|Tested Species reactivity||Human, Mouse, Non-human primate, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: C(157) T K A H H S S Q E M S S E Y R E(172)|
|Storage buffer||tris with glycine, 1mg/ml BSA|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-545 detects ethanolaminephosphate cytidyltransferase (ECT) protein in human, rat, mouse and non-human primate samples.
PA1-545 has successfully been used in Western blot procedures. By Western blot, this antibody detects an ~50 kDa protein representing ECT from C3H/10T1/2 cell lysates.
PA1-545 immunizing peptide corresponds to amino acid residues 157-172 from human ECT protein. This immunizing peptide (PEP-241) can be used for neutralization and control experiments.
ECT (ethanolaminephosphate cytidyltransferase) is an important regulatory enzyme in the pathway for phosphatidylethanolamine (PE) biosynthesis. PE functions in cell signalling, membrane fusion, platelet activation, and cell cycle progression. Specialized forms of PE exist as plasmologens, natural cannabinoid anadimade, and glycosylated PE. The ECT enzyme acts in the second of three catalysis steps to produce CDP-ethanolamine from phosphoethanolamine (P-Etn). Study of ECT could help determine the role of plasmologens in disease, specifically lipid disorders such as peroxisomal diseases as well as neuronal degeneration associated with Alzheimer's disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CTP:phopshoethanolamine cytidylyltransferase; CTP:phosphoethanolamine cytidylyltransferase; Ethanolamine-phosphate cytidylyltransferase; Ethanolaminephosphate Cytidyltransferase; Phosphorylethanolamine transferase
Gene Aliases: 1110033E03Rik; ET; PCYT2
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