|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||See 7 publications below|
|ChIP assay (ChIP)||See 2 publications below|
|ELISA (ELISA)||See 1 publications below|
|Immunoprecipitation (IP)||See 21 publications below|
|Immunocytochemistry (ICC)||See 3 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Rat , Non-human primate , Hamster , Human , Mouse|
|Host / Isotype||Mouse / IgG2a|
|Clone||528 + 199.12|
|Immunogen||Partially purified EGFR from A431 cells (clone 528) and extracellular domain of recombinant human EGFR protein (clone 199.12)|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-13697 targets Epidermal Growth Factor Receptor in IP applications and shows reactivity with Human samples.
The MA5-13697 immunogen is partially purified EGFR from A431 cells (clone 528) and extracellular domain of recombinant human EGFR protein (clone 199.12).
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; epidermal growth f; Epidermal growth factor receptor; erb-b2 receptor tyrosine kinase 1; ERBB; ERBB1; HER1; mENA; PIG61; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
Gene Aliases: EGFR; ERBB; ERBB1; HER1; mENA; NISBD2; PIG61
UniProt ID: (Human) P00533
Entrez Gene ID: (Human) 1956
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