|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 121 and 347 of Human EIF3A|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-31296 targets EIF3A in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-31296 immunogen is recombinant fragment corresponding to a region within amino acids 121 and 347 of Human EIF3A.
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: centrosomin homolog; cytoplasmic protein p167; eIF-3-theta; EIF3; eIF3 p167; eIF3 p180; eIF3 p185; EIF3, p180 subunit; eIF3-p170; eIF3-theta; EIF3A; EIF3S10; eukaryotic translation initiation factor 3; Eukaryotic translation initiation factor 3 subunit 10; Eukaryotic translation initiation factor 3 subunit A; eukaryotic translation initiation factor 3, subunit 10 (theta, 150/170kD); eukaryotic translation initiation factor 3, subunit 10 (theta, 170kD); eukaryotic translation initiation factor 3, subunit 10 theta, 150/170kDa; eukaryotic translation initiation factor 3, subunit 10, 170kD; eukaryotic translation initiation factor 3, subunit A; P167; p180 subunit; TIF32
Gene Aliases: EIF3; eIF3-p170; eIF3-theta; EIF3A; EIF3S10; KIAA0139; P167; p180; p185; TIF32
UniProt ID: (Human) Q14152
Entrez Gene ID: (Human) 8661
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