|Western Blot (WB)||1:1000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 32-61 amino acids from the N-terminal region of human EIF4E|
|Purification||Size-exclusion - Dialysis, Ammonium sulfate precipitation|
|Contains||0.09% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This antibody is predicted to react with bovine, mouse, rat, rabbit and Xenopus and zebrafish based on sequence homology.
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. this gene also interacts mutually exclusive with EIF4A1 and EIF4A2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: eIF-4E; eIF-4F 25 kDa subunit; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E-like 1; mRNA cap-binding protein
Gene Aliases: AUTS19; CBP; eIF-4E; EIF4E; EIF4E1; EIF4EL1; EIF4F
UniProt ID: (Human) P06730
Entrez Gene ID: (Human) 1977
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