|Immunoprecipitation (IP)||2 ug|
|Western Blot (WB)||0.01 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant human protein purified from E.coli (His-NNE)|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
A suggested positive control for this product is A431 cell lysate.
Neuron specific enolase (NSE) is an enzyme which catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. It is one of three mammalian enolases, which are also known as ENO1, ENO2, and ENO3 or alternately as enolase alpha, beta and gamma. The three enolases have different cell type specific expression patterns, so that antibodies to them are useful cell type specific markers.(MacAlesse et al., 1988). NSE corresponds to ENO2 or enolase gamma and is heavily expressed in neuronal cells. ENO1 is also known as enolase alpha and as non-neuronal enolase. The third enolase, ENO3 or enolase beta, is expressed in muscle cells. Since neurons require a great deal of energy, they are very rich in glycolytic enzymes such a GAPDH and NSE. Antibodies to this protein are therefore useful to identify neuronal cell bodies, developing neuronal lineage and neuroendocrine cells. Release of NSE from damaged neurons into CSF and blood has also been used as a biomarker of neuronal injury.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 2-phospho-D-glycerate hydro-lyase; alpha enolase like 1; Alpha-enolase; C-myc promoter-binding protein; ENO1L1; Enolase 1; enolase 1, (alpha); enolase-alpha; epididymis secretory protein Li 17; MBP-1; MPB-1; MPB1; MYC promoter-binding protein 1; NNE; Non Neuronal Enolase; Non-neural enolase; Phosphopyruvate hydratase; Plasminogen-binding protein; PPH; tau-crystallin
Gene Aliases: ENO1; ENO1L1; HEL-S-17; MBPB1; MPB1; NNE; PPH
UniProt ID: (Human) P06733
Entrez Gene ID: (Human) 2023
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