|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from internal region of human ERAB protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil or Alzheimer's brain tissue.
Mutations in several genes associated with early onset Alzheimer and quote;s result in increased extracellular concentrations of the longer form of the beta-amyloid peptide Ab 1-42 relative to Ab 1-40. It is this longer form of Ab that has been shown to be toxic to neurons and may serve as a catalyst for the aggregation and deposition of Ab to produce the neurotoxic effects associated with senile plaque formation. Using the Ab peptide in a yeast two-hybrid screen, a novel interacting protein designated the endoplasmic reticulum-associated amyloid beta-peptide-binding protein (ERAB)/L-3-hydroxyacyl-CoA dehydrogenase type II has been identified. It is shown to be expressed at high levels in Alzheimer and quote;s disease-affected brain. ERAB may contribute to neuronal dysfunction in Alzheimer and quote;s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 17-beta-HSD 10; 17-beta-hydroxysteroid dehydrogenase 10; 17b-HSD10; 3-hydroxy-2-methylbutyryl-CoA dehydrogenase; 3-hydroxyacyl-CoA dehydrogenase type II; 3-hydroxyacyl-CoA dehydrogenase type-2; AB-binding alcohol dehydrogenase; ABAD; amyloid-beta peptide binding alcohol dehydrogenase; CAMR; DUPXp11.22; Endoplasmic reticulum-associated amyloid beta-peptide-binding protein; ERAB; HADH2; HCD2; hydroxysteroid (17-beta) dehydrogenase 10; hydroxysteroid dehydrogenase 10; MHBD; Mitochondrial ribonuclease P protein 2; mitochondrial RNas; Mitochondrial RNase P protein 2; mitochondrial RNase P subunit 2; MRPP2; MRX17; MRX31; MRXS10; SCHAD; SDR5C1; Short chain dehydrogenase/reductase family 5C member 1; short chain dehydrogenase/reductase family 5C, member 1; short chain L-3-hydroxyacyl-CoA dehydrogenase type 2; short chain type dehydrogenase/reductase XH98G2; Short-chain type dehydrogenase/reductase XH98G2; Type II HADH
Gene Aliases: 17b-HSD10; ABAD; CAMR; DUPXp11.22; ERAB; HADH2; HCD2; HSD17B10; MHBD; MRPP2; MRX17; MRX31; MRXS10; SCHAD; SDR5C1; XH98G2
UniProt ID: (Human) Q99714
Entrez Gene ID: (Human) 3028
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