|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay dependent|
|Western Blot (WB)||1:1000|
|Western Blot (WB)||See 2 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues F(159) D S F D N D G K K N N P A I(173) of rat ERGIC-53.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-074 detects ERGIC-53 in rat, mouse and human samples.
PA1-074 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a ~57.5 kDa protein representing ERGIC-53 in rat brain samples.
The PA1-074 immunogen is a synthetic peptide corresponding to residues F(159) D S F D N D G K K N N P A I(173) of rat ERGIC-53. This sequence is conserved in mouse and human. The PA1-074 immunizing peptide (Cat. # PEP-277) is available for use in neutralization and control experiments.
Protein trafficking along the endoplasmic reticulum (ER) to the Golgi apparatus occurs by vesicular transport between membranous compartments. ERGIC-53 (ER-Golgi intermediate compartment 53) is a mannose-specific lectin transport receptor protein which cycles between the ER and the Golgi complex. ERGIC-53 is involved in intracellular transport of many glycoproteins. ERGIC-53 is useful in studying mechanisms underlying protein trafficking early in the secretory pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: endoplasmic reticulum-golgi intermediate compartment protein 53; ER-Golgi intermediate compartment 53 kDa protein; Gp58; Intracellular mannose-specific lectin MR60; Lectin mannose binding-1; Lectin mannose-binding 1; lectin, mannose-binding, 1; Lman1; p58; Protein ERGIC-53
Gene Aliases: 2610020P13Rik; AI326273; AU043785; C730041J05; ERGIC-53; ERGIC53; F5F8D; FMFD1; gp58; LMAN1; MCFD1; MR60; P58
Molecular Function: membrane traffic protein
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.