|Western Blot (WB)||1-3 µg/ml|
|Tested Species reactivity||Bovine, Human, Mouse, Rat|
|Host / Isotype||Goat / IgG|
|Immunogen||synthetic peptide EEKPKKKKKAQEDL, corresponding to C terminal amino acids 492-505 of Human ERp57.|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA1-31316 detects ERp57 from human samples.
PA1-31316 has been successfully used in Western blot and ELISA applications.
The PA1-31316 immunogen is: Synthetic peptide: EEKPKKKKKAQEDL, corresponding to C terminal amino acids 492-505 of Human ERp57.
ERp57, also known as GRP58, was cloned by three independent labs, all of which reported that the gene encodes a 505-amino acid protein that shares significant homology with the molecular chaperone protein disulfide isomerase (PDI). The ERp57 sequence was also shown to include a putative nuclear localization motif and an endoplasmic reticulum (ER) retention motif. Studies have shown that ERp57 functions together with calnexin and calreticulin as a molecular chaperone of glycoprotein biosynthesis within the ER.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 58 kDa glucose-regulated protein; 58 kDa microsomal protein; Disulfide isomerase ER-60; endoplasmic reticulum P58; endoplasmic reticulum protein; Endoplasmic reticulum resident protein 57; Endoplasmic reticulum resident protein 60; epididymis secretory protein Li 269; epididymis secretory sperm binding protein Li 93n; ER protein 57; ER protein 60; ER-60 protease; ER60; ERp57; ERP60; ERp61; glucose regulated protein 58kD; glucose regulated protein, 58 kDa; glucose regulated protein, 58kDa; GRP57; GRP58; HIP-70; HsT17083; oxidoreductase ERp57; p58; phospholipase C, alpha; phospholipase C-alpha; PI-PLC; protein disulfide isomerase associated 3; protein disulfide isomerase family A, member 3; protein disulfide isomerase-associated 3; Protein disulfide-isomerase A3; Q-2
Gene Aliases: 58kDa; BOS_20460; ER60; Erp; ERP57; ERP60; ERp61; GRP57; GRP58; HEL-S-269; HEL-S-93n; HsT17083; P58; PDI; PDI-Q2; PDIA3; PI-PLC; PLC[a]; Plca
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