|Western Blot (WB)||1/500 - 1/2000|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human ETV4 (aa50-109) expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA5-15424 targets ETV4 in WB applications and shows reactivity with Human samples.
The MA5-15424 immunogen is purified recombinant fragment of human ETV4 (aa50-109) expressed in E. Coli.
MA5-15424 detects ETV4 which has a predicted molecular weight of approximately 54kDa.
ETV4: ets variant gene 4 (E1A enhancer binding protein, E1AF), also known as PEA3. Several members of the Ets gene family are known to encode sequencespecific DNA binding proteins. These include mouse PU.1, mouse and human Ets-1, Drosophila E74, chicken and human Ets-2 and rat GABP-a. Each of these proteins recognizes similar motifs in DNA that share a centrally located 5'-GGAA-3' element. For instance, PEA3 binds the motif 5'-AGGAAG-3' (the PEA-3 motif), but does not bind to the sequence 5'-AGGAAC-3', recognized by PU.1, although PU.1 binds equally well to both sequences. It appears thatall of the Ets proteins recognize the same central core sequence but that each protein interacts with unique sequences that flank this core. PEA3 is expressed at readily detectable levels in cells of epithelial and fibroblastic origin but is not expressed in hematopoietic cells. This is in contrast to other members of the Ets gene family, such as Ets-1, Ets-2 and Fli-1, each of whichis expressed primarily in cells of hematopoietic origin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Adenovirus E1A enhancer-binding protein; E1A-F; E1AF; ETS translocation variant 4; ets variant gene 4 (E1A enhancer-binding protein, E1AF); ETV4; EWS protein/E1A enhancer binding protein chimera; PEA3; PEAS3; Polyomavirus enhancer activator 3 homolog; Protein PEA3
Gene Aliases: E1A-F; E1AF; ETV4; PEA3; PEAS3
UniProt ID: (Human) P43268
Entrez Gene ID: (Human) 2118
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