|Western Blot (WB)||1:250|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the C-terminal residues R(1821) P E L Q E K A A L L K K T L E N L E(1839) of human Ecm29.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA3-035 detects Ecm29 in human and mouse samples.
PA3-035 has been used successfully in Western blot and immunofluorescence procedures. In Western blot analysis of HeLa cell extracts this antibody detects a ~205 kDa protein representing Ecm29.
The PA3-035 immunogen is a synthetic peptide corresponding to the C-terminal residues R(1821) P E L Q E K A A L L K K T L E N L E(1839) of human Ecm29.
Ecm29 appears to be one of three proteins which associate significantly with proteasomes. Ecm29 has been shown to enhance the stability of the proteasome. Ecm29 deletion has been shown to produce 26S proteasomes which dissociated in the absence of ATP. It was thus suggested that Ecm29 tethers the regulatory complex to the proteasome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Ecm29; ECM29 homolog, proteasome accessory protein; FLJ22036; homolog of yeast Ecm29; KIAA1962; Proteasome associated protein ECM29 homolog; Proteasome-associated protein ECM29 homolog; RP11-386D8.2
Gene Aliases: AW558785; BB181316; ECM29; KIAA0368; mKIAA0368
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.