|Flow Cytometry (Flow)||0.5-2ug per test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:100-1:500|
|Immunohistochemistry (IHC)||See 27 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Western Blot (WB)||See 2 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Human , Mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Synthetic Peptide: C R(1250) S T L Q H P D Y L Q E Y S T(1264)|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-861 detects Her-4 from human and mouse samples.
MA1-861 has been successfully used in immunoprecipitation, immunohistochemical, and Western blotting procedures. By Western blot, this antibody detects ~185 kDa protein representing Her-4 from human A431 cell and mouse NIH3T3/HER4 cell extracts.This antibody has also been used in immunohistochemical, using paraffin embedded mouse NIH3T3 cells.
MA1-861 immunizing peptide corresponds to amino acid residues 1250 to 1264 of human Her-4 oncoprotein.
Her-4, also known as c-erbB-4 and v-erbB-4, is a Type 1 membrane protein found in numerous cell types. It is a member of the EGF receptor protein family, and is activated by neuregulins and NTAK. Her-4 levels have been found to be elevated in certain human tumor cell lines suggesting that it may play a role in some malignancies. Her-4 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4; Avian erythroblastic leukemia viral (verbb2) oncogene homolog 4; avian erythroblastosis oncogene B 4; C-erbB-4; ERBB4; ERBB4 transcript variant I12DEL; ERBB4 transcript variant I20DEL; human epidermal growth factor receptor 4; p180erbB4; Proto-oncogene-like protein c-ErbB-4; Receptor tyrosine-protein kinase erbB-4; Tyrosine kinase type cell surface receptor HER4; Tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4; Verb a e
Gene Aliases: ALS19; c-erbB-4; ERBB4; HER4; Mer4; p180erbB4
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