|Immunohistochemistry (Paraffin) (IHC (P))||10-20 µg/mL|
|Immunoprecipitation (IP)||10 µg/ml|
|Western Blot (WB)||2.5-5.0 µg/ml|
|Western Blot (WB)||See 2 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Tested Species reactivity||Bovine, Human, Mouse, Sheep, Pig, Rabbit, Rat|
|Published species reactivity||Rat Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide from the N-terminus of human estrogen receptor, alpha protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
PA5-16476 targets Estrogen Receptor in IF, IHC (P), IP, and WB applications and shows reactivity with mouse, Rabbit, Rat, Bovine, Human, Ovine, and Porcine samples.
The PA5-16476 immunogen is a synthetic peptide from the N-terminus of human estrogen receptor, alpha protein.
Estrogen Receptors (ER) are members of the steroid family of nuclear receptors. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Each protein contains distinct functional domains required for transcriptional activation, binding to estrogen response elements (ERE) in DNA, constitutive dimerization, binding to heat shock proteins, and ligand recognition. ER is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to EREs. When bound to DNA, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are several different isoforms of both ER alpha and ER beta. ER is an important regulator of growth and differentiation in the mammary gland. The presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ER; Er alpha; ER-alpha; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor 1 (alpha); estrogen receptor alpha; estrogen receptor alpha c-terminus splice variant 1-2; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; estrogen receptor alpha variant delta 4; estrogen receptor beta; estrogen receptor beta 4; estrogen receptor protein; Nuclear receptor subfamily 3 group A member 1; nuclear receptor subfamily 3 group A member 2
Gene Aliases: BOS_9943; ER; ER-alpha; ERa; ERalpha; ESR; ESR1; ESRA; Estr; Estra; ESTRR; NR3A1; RNESTROR
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