|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Western Blot (WB)||1:25|
|Miscellaneous PubMed (MISC)||See 3 publications below|
|Western Blot (WB)||See 23 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 18 publications below|
|Immunocytochemistry (ICC)||See 6 publications below|
|Immunohistochemistry (IHC)||See 193 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Rat , Virus , Sheep , Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminus of human estrogen receptor alpha|
|Storage buffer||tissue culture supernatant diluted in TBS, pH 7.5, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody is predicted to react with porcine based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is breast carcinoma.
The human estrogen receptor (ER) exists in two forms, ERa and ERß. The receptors have a high degree of homology in their DNA binding and ligand binding domains and can form heterodimers. However, the two ERs signal in different ways; for example, ERß does not potentiate the nuclear transcription factor AP-1 when complexed with oestradiol, whereas ERa does. This suggests that ERa and ERß may have different roles in gene regulation and their relative levels within tissues may influence cellular responses to oestrogens.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DKFZp686N23123; ER; ER-alpha; Era; ESR; ESRA; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; NR3A1; Nuclear receptor subfamily 3 group A member 1; RP1-130E4.1
Gene Aliases: ER; Era; ESR; ESR1; ESRA; ESTRR; NR3A1
UniProt ID: (Human) P03372
Entrez Gene ID: (Human) 2099
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