|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:400|
|Western Blot (WB)||1:200-1:500|
|Western Blot (WB)||See 25 publications below|
|Miscellaneous PubMed (MISC)||See 3 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 23 publications below|
|Immunohistochemistry (IHC)||See 199 publications below|
|Immunocytochemistry (ICC)||See 7 publications below|
|Immunoprecipitation (IP)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Rat , Virus , Sheep , Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from C-terminal of human estrogen receptor|
|Storage buffer||tissue culture supernatant|
|Contains||15mM sodium azide|
|Storage conditions||4° C|
This antibody strongly stains the nucleus of epithelial cells in breast carcinomas. It reacts with ER alpha, 67 kDa. No special pretreatment is required for IHC staining.
Estrogen Receptors (ER) are members of the steroid family of nuclear receptors. There are two different forms of the estrogen receptor, alpha and beta, encoded by separate genes (ESR1 and ESR2, respectively). Each protein contains distinct functional domains required for transcriptional activation, binding to estrogen response elements (ERE) in DNA, constitutive dimerization, binding to heat shock proteins, and ligand recognition. ER is a ligand-activated transcription factor, that when bound to estrogen hormone, induces a conformational change that allows dimerization and binding to EREs. When bound to DNA, ER can positively or negatively regulate gene transcription through the recruitment of coactivator or corepressor proteins. There are several different isoforms of both ER alpha and ER beta. ER is an important regulator of growth and differentiation in the mammary gland. The presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ER; 4; 5; 6; 7*/654 isoform; 7*/819-2 isoform; ER-alpha; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor alpha delta 3*; estrogen receptor alpha delta 4 +49 isoform; estrogen receptor alpha delta 4*; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; Nuclear receptor subfamily 3 group A member 1
Gene Aliases: ER; Era; ESR; ESR1; ESRA; ESTRR; NR3A1
UniProt ID: (Human) P03372
Entrez Gene ID: (Human) 2099
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