|Immunohistochemistry (Paraffin) (IHC (P))||0.4-0.8 µg/mL|
|Western Blot (WB)||0.5-1.0 µg/ml|
|Immunohistochemistry (IHC)||See 53 publications below|
|Western Blot (WB)||See 5 publications below|
|Immunocytochemistry (ICC)||See 3 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Rabbit , Rat , Cat , Human , Mouse , Chicken , Rhesus monkey Guinea pig|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human estrogen receptor protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissues in 10mM citrate buffer (pH 6.0) for 10-20 min, followed by cooling at room temperature for 20 min. A recommended positive control is MCF-7 cells. For optimal IF staining, it is recommended to fix and permeabilize cells with methanol.
The Estrogen Receptor alpha (ER alpha) protein is approximately 66kD, though multiple isoforms ranging from 36-66kD have been described. The ER alpha gene (ESR1) consists of more than 140kb of genomic DNA divided into 8 exons, being translated into a protein with six functionally discrete domains, labeled A through F. Each domain is functionally distinct, required for transcriptional activation, binding to estrogen response elements (ERE) in DNA, constitutive dimerization, binding to heat shock proteins, and ligand recognition. ER is an important regulator of growth and differentiation in the mammary gland. The presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.
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Protein Aliases: ER; 4; 5; 6; 7*/654 isoform; 7*/819-2 isoform; ER-alpha; Estradiol receptor; estrogen nuclear receptor alpha; Estrogen receptor; estrogen receptor alpha delta 3*; estrogen receptor alpha delta 4 +49 isoform; estrogen receptor alpha delta 4*; estrogen receptor alpha E1-E2-1-2; estrogen receptor alpha E1-N2-E2-1-2; estrogen receptor alpha splice variant, CTERP-1; estrogen receptor alpha splice variant, ERalphaDup5; estrogen receptor alpha splice variant, ERalphai45a; estrogen receptor alpha splice variant, ERalphai45bL; estrogen receptor alpha splice variant, ERalphai45bS; estrogen receptor alpha splice variant, ERalphai45c; estrogen receptor alpha splice variant, ERalphai56; estrogen receptor alpha splice variant, ERalphai67; Nuclear receptor subfamily 3 group A member 1
Gene Aliases: ER; Era; ESR; ESR1; ESRA; ESTRR; NR3A1
UniProt ID: (Human) P03372
Entrez Gene ID: (Human) 2099
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